Progress of epithelial-mesenchymal transition in respiratory system and the modulatory mechanism of cell adhesion / 生理学报

Epithelial-mesenchymal transition (EMT) plays an important role in the development and pathogenesis of respiratory system. Epithelial cells are characterized by well-developed, intercellular contacts, whereas EMT triggers the sequential destabilization of cell-cell adhesive junctions. The dynamic remodeling of the epithelial cell adhesion molecules is important for maintaining the integrity and normal function of epithelium. This paper reviews the research progress of EMT in lung development, lung injury repair and chronic lung diseases, and summarizes the effect of cell junctions and cell adhesion molecules on EMT molecular events.

Quality stability of Ligustri Lucidi Fructus / 中国药学杂志

OBJECTIVE: To study the change rule of specnuezhenide content and antoxidant activity of Ligustri Lucidi Fructus with temperature and time, and determine its validity period. METHODS: Dynamic parameters were established under constant temperature, and specnuezhenide content of Ligustri Lucidi Fructus was determined by HPLC after accelerated test by classical constant temperature method. The shelf life of Ligustri Lucidi Fructus at 25℃ were determined by Arrhenius theory. The antioxidant activities of different samples of Ligustri Lucidi Fructus were evaluated by ABTS assay. RESULTS: The shelf life of Ligustri Lucidi Fructus was forecasted to be 1.83 years at 25℃. The antioxidant activitiy of Ligustri Lucidi Fructus showed a trend of decline after rising first. CONCLUSION: High temperature and storage time would affect the chemical composition of Ligustri Lucidi Fructus, so Ligustri Lucidi Fructus should be kept in the shade for no more than 1.83 years for a good therapeutic effect.

Spectrum-Effect Relation of Antioxidant Activity of Flos Carthami Based on DPPH, ABTS and FRAP Assay / 中国药学杂志

OBJECTIVE: To study the active ingredients of Flos Carthami by investigating the relationship between HPLC spectrum and antioxidant activity. METHODS: The specific chromatogram of Flos Carthami processed by means of classical homothermal acceleration was assayed by HPLC. DPPH, ABTS and FRAP assays were established to determine the antioxidant activity of Flos Carthami. The spectrum-effect correlation was studied by partial least squares (PLS) method. RESULTS: S4, S8, S11, S12, S13, S14, S16 and S17 were characteristic compounds in 27 matching characteristic chromatograms which had positive relationship with the ability of scavenging DPPH free radical. S4, S8, S11, S12, S14, S16, S17 and S18 were significantly positively related to the ability of scavenging ABTS free radical. S4, S8, S11, S12, S13, S14, S16, S17, S18 and S26 were significantly positively related to the ability of restoring Fe3+. Among the chromatographic peaks, S4, S8, S11, S12, S14, S16and S17 were positively related to the abilities of scavenging DPPH free radical and ABTS free radical and restoring Fe3+. S14 was determined as hydroxysafflor yellow A. CONCLUSION: The antioxidant material of Flos Carthami is based on the synergy of a variety of compounds. An as comprehensive as possible analysis of the compounds related to the pharmacological effect can reflect the quality of Flos Carthami. The study of spectrum-efficacy relationship is an effective way to determine the compounds related with pharmacological effect and control the quality of traditional Chinese medicinal materials.

Spectrum-effect relationship in antioxidant activity of Ligustri Lucidi Fructus based on DPPH, ABTS and FRAP assays / 中国中药杂志

Characteristic chromatogram of Ligustri Lucidi Fructus which were processed by heat treatment with different temperatures and times was developed by HPLC. DPPH, ABTS and FRAP assays were performed to determine the antioxidant activity. The spectrum-effect correlation was studied using Partial Least Squares(PLS)to explore the active ingredients of Ligustri Lucidi Fructus by investigating the relationship between HPLC spectrum and antioxidant activity. S1, S2, S15, S24, S27, S29, S32, S33 and S35 were characteristic compounds in 35 matching characteristic chromatograms which were positive relationship with scavenging DPPH free radical activity. S1, S2, S5, S8, S16, S27 and S33 were significantly positively related to scavenge ABTS free radical activity. S12, S20, S22, S28, S30, S31, S32 and S34 were significantly positively related to restore Fe³⁺ activity. Among the chromatographic peaks, S1, S2, S27 and S33were positively related to scavenge DPPH free radical and ABTS free radical activity, and S32 was positively related to scavenge DPPH free radical and restore Fe³⁺ activity. S3, S19, S21, S22, S23, S33 and S34 were determined as salidroside, luteolin-7-O-glucoside, specnuezhenide, oleuropein, ligustroflavone, luteolin andapigenin, respectively. The results showed that although specnuezhenide and salidroside were stipulated index compounds to evaluate the quality of Ligustri Lucidi Fructus, the Ligustri Lucidi Fructus pharmcodynamic effect did not depend on the contents of those index components. The quality of traditional Chinese medicines(TCMs)should be determined by the compound groups associated with pharmcodynamic effect.

Research progress on chemical compounds and pharmacological effects of Caesalpinia / 中国中药杂志

Consult literature, the chemical composition of the type of system Caesalpinia plants were summarized and discussed in detail its pharmacological effects. The genus contains major chemical components of high isoflavones, chalcones and diterpenoids, pharmacological effects inhibit melanin production, anti-inflammatory, antioxidant, antibacterial, immune regulation, and further research in this genus offer reference.

Effect of heating treatment on stability of two coumarins in Angelica dahurica and activity of tyrosinase / 中国中药杂志

To study the heating treatment of Angelica dahurica under different temperature and time conditions on the stability of coumarins and tyrosinase activity. HPLC method was used to determine the contents of imperatorin and isoimperatorin, and tyrosinase activity was assayed by measuring the oxidation rate of L-DOPA in vitro. After heated, the contents of imperatorin increased. Expect for being heated at 90 ℃ for 2 h, the content of isoimperatorin was higher than crude one. Before and after being heated, A. dahurica showed an activating effect on tyrosinase. In the same temperature and time conditions, the activation rate increased with the rise of concentration of tyrosinase extracts. Heating process for A. dahurica could change the contents of imperatorin and isoimperatorin, mainly increasing their concentrations.

Spectrum-effect relationship of antioxidant activity of wine fried ligustrum lucidum fructus / 中国药学杂志

OBJECTIVE: To explore the active ingredients of wine fried Ligustrum lucidum fructus by studying the relationship between the HPLC chromatogram and antioxidant activity. METHODS: The characteristic chromatogram of wine fried Ligustrum lucidum fructus processed by means of classical homothermal acceleration was established by HPLC. DPPH, ABTS, and FRAP methods were established to determine the antioxidant activity. The spectrum-effect relationship was studied by using partial least squares regression (PLSR), and the chromatographic peaks related to antioxidant activity were identified. RESULTS: Peaks 1, 3, 8, 10, 14 and 15 were found to have positive relationship with DPPH free radical and ABTS free radical scavenging activity among the 17 matching characteristic chromatograms. Peaks 3, 8, 12, and 13 were significantly positively related to Fe3+ resuction capacity. Among the chromatographic peaks, peak 3 and peak 8 were positively related to the activities of scavenging DPPH free radical and ABTS free radical and reducing Fe3+. Peaks 3, 12, 13, 14, 15, and 17 were determined as salidroside, luteolin-7-O-glucoside, specnuezhenide, oleuropein, ligustroflavone, and luteolin, respectively. CONCLUSION: The pharmcodynamic effects of wine fried Ligustrum lucidum fructus do not depend on the contents of several index components such as specnuezhenide and salidroside. The quality of traditional Chinese medicines should be represented by the compound groups associated with pharmcodynamic effect.

Determination of effective time of Schizonepeta tenuifalia pieces by classical constant temperature acceleration experiment / 中国中药杂志

<p><b>OBJECTIVE</b>To study the validity of Schizonepeta tenuifalia pieces.</p><p><b>METHOD</b>The change principle for the contents of essential oil and pulegone was determined by the way of classical constant temperature acceleration experiment, and the reacting speed at 20 degrees C was calculated according to Arrhenius index law and effective time was calculated.</p><p><b>RESULT</b>In classical constant temperature experiment, the content change of essential oil with the regular pattern of one level. The effective time of S. tenuifalia pieces stored in 20 degrees C was 2.08 years and the constant of speed is 8.453 4 x 10(-6) carried out by essentialoil, 190 days with the constant of speed is 3.39 x 10(-5) carried out by pulegone.</p><p><b>CONCLUSION</b>The effective time of S. tenuifalia pieces was about 2 years carried out by essential oil and 190 days carried out by pulegone by the way of classical constant temperature experiment.</p>

Stable life predection for honeysuckle by initial average rate stability test / 中国中药杂志

<p><b>OBJECTIVE</b>To study the content change of chlorogenic acid in honeysuckle under different temperature, and predict its term of validity at room temperature.</p><p><b>METHOD</b>The content of chlorogenic acid was assayed by HPLC. The activation energy E(a) and the decomposition rate constant K were calculated by initial average rate stability tests and the prediction was carried out.</p><p><b>RESULT</b>The stable life of honeysuckle has been determined as 1.29 years.</p><p><b>CONCLUSION</b>The initial average rate stability test was used to predict stable life of honeysuckle, and the results are credible. Higher temperature should be avoided for honeysuckle store, and room temperature should guarantee its quality during storage.</p>

Stable life predection for Mentha haplocalyx by initial average rate stability test / 中国中药杂志

<p><b>OBJECTIVE</b>To predict the stable life for Mentha haplocalyx.</p><p><b>METHOD</b>The volatiles in M. haplocalyx were analyzed by head-space solid micro-extraction, coupled with GC-MS and a comprehensive evaluation of essential oil in M. haplocalyx was analyzed using the factor analysis. The prediction was carried out by initial average rate stability tests using the content of essential oil and the main volatiles as indices.</p><p><b>RESULT</b>Principal component analysis indicated that pulegone and isomenthone can fully describe the quality of prepared slices. The t(0.9, 20 degrees C) was 5.49 years and 2.88 years respectively, carried out by essential oil, pulegone and isomenthone.</p><p><b>CONCLUSION</b>The stable life for M. haplocalyx under 20 degrees C was 2.88 years.</p>

Analysis of volatile constituents in two species of genus Magnolia by HS-SPME-GC-MS / 中国中药杂志

<p><b>OBJECTIVE</b>To analyze and compare the volatile constituents from M. biondii and M. liliflora.</p><p><b>METHOD</b>The volatile constituents were extracted by head-space solid-phase microextraction, and analyzed by GC-MS.</p><p><b>RESULT</b>Seventy two constituents were identified from M. biondii and M. liliflora, the content of the 25 constituents in both samples were similar, while the kinds of the constituents were obviously different.</p><p><b>CONCLUSION</b>The volatile constituents were different between M. biondii and M. liliflora.</p>

Terpenoids from leaves of Aeschynanthus mengxinensis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the ethyl acetate portion of an ethanolic extractive of the leaves of Aeschynanthus mengxinensis.</p><p><b>METHOD</b>The column chromatographic techniques were applied to isolate constituents. A combination of IR, ESI-MS, NMR and 2D-NMR spectroscopy was used to identify structures.</p><p><b>RESULT</b>Four compounds were isolated from the ethyl acetate fraction of this plant, and the structure of them have identified as 2alpha, 3beta, 19beta-trihydroxyolean-12-ene-23,28-dioic acid (1), 2alpha, 3beta, 21beta-trihydroxyolean-12-ene-28-oic acid (2), 2alpha, 3beta, 23-trihydroxyurs-12-ene-28-oic acid (3) and stigmast-5 (6), 22 (23)-diene-3beta-ol (4).</p><p><b>CONCLUSION</b>The NMR data of compound 1 was completely assigned by 2D-NMR techniques, including HMBC and HMQC. Compounds 1-4 were isolated for the first time from Gesneriaceae.</p>